In vivo ensemble recording with tetrodes

Behavioral neurophysiology has been the workhorse of system neuroscience for many decades. With the introduction of multi-electrode array, especially the configuration of tetrode drives, tens or even hundreds of cells can be recorded simultaneously.

Behavioral Neurophysiology
Rat with Hyperdrive
Spike Sorting

Molecular and viral tools

Viral technologies have revolutionized modern neuroscience. With the ability to introduce almost arbitrary endogenous or exogenous genetic element in cells with anatomical/genetic/expression profiles, we are endowed with the ability to dissect and manipulate our circuits of interest.

Microendoscopic calcium imaging in freely moving animals

High-through activity monitoring can also be achieved with calcium imaging. With the advent of miniaturized devices, it is now feasible to associate the activity of genetically defined cells with behavior.

Mice with Miniscope
Recorded Cells

In vivo two-photon guided whole-cell patch/cell-attached recording, juxtacellular labeling and recording in freely moving animals

Although it’s convenient to be able to access the activity of an ensemble of neurons, we are loosing important details, such as the morphology, location, and local connectivity properties. The tried-and-tested traditional techniques (with modern modifications) still shine in various applications.

A Hippocampal Pyramidal Cell

Computational data analysis and modeling

We use various computational techniques to model and interpret our experimental data.